nlwxpearo
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 Posted: Mon 10:30, 24 Jan 2011 Post subject: According to hybrid sterility locus S-5 ^ n allele |
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According to hybrid sterility locus S-5 ^ n allele predicted PCR markers indica rice / japonica hybrid success
Difficult to distinguish the size of the Indica and the formation of a Java-based banding (Figure 1, omitted). Therefore, when the need to transfer to the S-5, temperate japonica rice varieties suitable when the tag is Taiwan; on this occasion should be a test cross of Japonica / Java type / / indica. When the hybrid access Indica / Java type / / When the order japonica, STS213-/STS213i,[link widoczny dla zalogowanych], STS213/STs2l3 and STS213 10 view of India as pointed out that the genetic background of strains in some time, should take advantage of RFLP clone RG213 (from one to the other identified sites of sterility may have derived our primer) or the existing chain of STS mark in these strains. They study of infertility is also recorded as the probe in Southern blot analysis, as they used the QTL analysis, the results s 5 illustrates a phenotypic marker to identify variable regions in the S-5, all three alleles of the opposite sex 45%. However, in our model from the glaze turn to the japonica gene for c shift of the question. STS213 marker predictable base of wide compatibility and sterility loci S-5 marker is valuable because of type,[link widoczny dla zalogowanych], is also conducive to identification is likely to result in hybridization,[link widoczny dla zalogowanych], because the phenotype can be accurately evaluated by the environment is sterile West orderly and those offspring plants: impact of genetic background as STS21.3. resulting in a 5 s difficult to use in breeding is a need for a small amount of DNA The PCR based markers, which the fertility of those plants. These same factors make it to the genotype of seedlings after the germination l-2 days OK. inevitability of our pre-alleles based on STS213 so on. STS213 benefit distant hybridization testing in the early selection of plants TCI Fertilities results confusing. this strain. unnecessary test cross, selfing, and most kinds of problems recorded by the group Yanagihara et al (1995) reported; plant fertility. They point to the S-5 group were RG213 mapping tease Xie translated CropScience37 (6), 1910 found in one continuous distribution of fertility rather than fertility 1912.1997 plants, ~ a, well Qu Hong shabu saying flower delivery, I,, through the medium modified to improve the efficiency of rice anther culture. Star: Triple Zheng Jia sKR1NA compensation, etc. - compared to a 1 and japonica rice,[link widoczny dla zalogowanych], indica rice anther culture of microspores produced by green plants Brown List usually minimal. using four indica �� enamel F-hybrid model as the test material, containing a higher amount of KN03 (31raM) and casein hydrolyzate (CH, 500mg / t) and the improvement of anther without ammonium medium with a wide range of raw materials used in the medium prime medium N compared test. of indica �� japonica and indica �� F-generation of Java-based hybrids was also studied. Materials and Methods anther donor plants (omitted) Test 1: This study conducted from March to June 1988 and anther culture plate in 3 months and 4 month. preliminary results show that in the presence of higher (3lmM) KNO3 salts but not the amount, and CH MS salt medium induced microspore callus callus than in the higher frequency of green plant regeneration. So. on the code 4 SK-1 medium and have N6N prime medium (MSN ) for a test (see Table 1) the same two media are complementary elements of organic N, and with a D. NAA and 2.4 K of the plant growth hormone. Test 2:1988 in June for anther culture plate . MSN does not contain any medium used to induce CH 4 F-hybrids microspore callus (Table 2 omitted). N6N cable containing materials MSN reason is that most of the researchers on the japonica / Java type cultivar preference for N6 basic medium. However, because our previous studies have shown that high (2mg / 1) 2,[link widoczny dla zalogowanych],4-D supplemented medium induces N6 and Java japonica rice cultivars of microspore callus overgrowth, resulting in very low power of green plants again. So just to maintain the original level and no added ammonium cH In addition, MSN with low levels of 2,4 D (0.? mg / 1) and K (075rag / 1). The purpose of these improvements is to make the resulting microspore callus higher than the 2.4-D supplement from the medium group of regular callus
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