nlwxpearo
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 Posted: Sat 23:05, 19 Feb 2011 Post subject: High-density gene chip technology and its developm |
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High-density gene chip technology and its development trend
Protection, the 3 'end hydroxyl groups were activated single nucleotide dNTP, dNTP to the 3' end of the activity of the substrate hydroxyl condensation, wash not effective combination of dNTP. (D) replace the mask, repeat 【b) and (c), until the desired finish kneading needle array synthesis. Mask should be selected probe array based on the required production needs. Synthesis in the film can play the advantages of microfabrication technology to achieve high-density chip scale preparation. Affymetrix has prepared the United States includes all 20 ~ 25met flutter nucleotide oligonucleotide probe arrays (6 ~ 40 million) of the chip, resolution of l0m. Therefore, in the film co-synthesis method is suitable for production of large-scale DNA CHlNA probe array chip. To sum up, began in the early 9o biochip research has been a good start, especially in the United States has developed many different products. In which p53 gene, HIV gene detection chip into the experimental stage. Taken together, research and production of bio-chip foreign-like j brother has the following characteristics: (I) Aflymelt'ix company has a bit of light off the cheaper synthetic preparation of patent protection technology, enabling the company to become a leader in the field of gene chips. Currently, the chip error rate in the synthesis of the remaining issues, the gene chips used only for research, the U.S. FDA for clinical standards have not yet humble. (2) most companies use print, jet printing, spotting, etc., the low-density DNA microarray technology. However, this technique is difficult to prepare high-density array-based chip, in a large-scale mass production and standardization of industry have certain difficulties. 3,[link widoczny dla zalogowanych], the current trend, the international development trend of bio-chips have the following characteristics: (1) from future large-scale mass production and application prospects point of view,[link widoczny dla zalogowanych], using high-resolution DNA synthesis in situ preparation of high-density gene chip is an important trends. Since deprotection in situ synthesis of light has been specifically protected Li, therefore, the development of new technologies such as molecular seal in situ DNA synthesis array will be a very important basic research topic. (2) high density in situ synthesis chip to the length of DNA probe molecules is limited. Therefore, preparation of high-density chip technology and the combination of DN segment ligation, preparation of large pieces of high-density DNA chips are an important part of the future. (3) the use of MEMS technology to develop high efficiency capillary array integrated microfluidic chip and corresponding system, and intelligent and automated microarray chip development will be one of the goals. Therefore,[link widoczny dla zalogowanych], we believe that our country should focus on high-density gene chip array. At the same time the development of bio-chip detection system. On this basis, the transport MEDICALDEVICESiNFORMATION16 with micro-mechanical technology,[link widoczny dla zalogowanych], the development of micro fluid analysis system, directly on the chip and other biological samples of cell nucleic acids, proteins and other biological molecules separation, extraction, analysis and amplification reactions developed Rapid Micro , Automatic, intelligent biochips and biochip used in genomic research, disease prevention and diagnosis, and drug design and screening. We proposed a ~ kind of high-density DNA microarray chip preparation, the method several times using molecular imprinting seal fixed in situ synthesis of DNA microarray preparation. The method of synthesis, low cost, combined with in situ synthesis rate, was prepared more than 60,000 gene probe array ~ (64K microarray) gene chip samples by hybridization with a given gene, the cause has been given encouraging results. Situ synthesis of the gene chip technology features: The very mature commercial DNA synthesis reagents, DNA probe synthesis, this would greatly save the cost of raw materials and R & D cycle. Single-step DNA synthesis to improve the yield of our single-step DNA chip yield more than 98.6%. Thus, can greatly improve the accuracy of DNA probe. Preparation of DNA chip seal elements can greatly simplify the preparation of bio-chip equipment investment, cost savings. The development of gene chips have been expected by many to support the efforts of 3 to 5 years,[link widoczny dla zalogowanych], the formation of a group of independent intellectual property patents for the development of a number of bio-chip prospect of sequencing the practical and diagnostic chips. High-density gene chip design, development and application are interrelated. Application of the development of gene chip research and clinical application prospects for the design of a large, high-performance high-density gene chip is very important. Bio-chip is the intersection of biology and electronics in a new concept, it's made of science and technology sector has attracted great attention and a high of enthusiasm. The broad application prospects also attracted industry attention. We believe that in the near future in the bio-chip of the research, development and application will also be a place in the international arena.
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