logoxgvb
Joined: 30 Aug 2010
Posts: 1580
Read: 0 topics
Warns: 0/5
Location: hdfttv
|
 Posted: Thu 14:04, 02 Dec 2010 Post subject: PUMA shoes wholesale Striped Bamboo shark liver re |
|
|
Striped Bamboo shark liver regeneration cDNA library construction and identification of plasmid
270 (4): 771 [10] Wei Jian Wen, Wu Yan, Zhong Xiaofen and so on. Marine functional genomics research. High-tech communications, 2OOO, 10 (10): 85lllJMichalopoulosGK,[link widoczny dla zalogowanych], DeFrancesM. Liverregeneration. Sc / one. 1997,[link widoczny dla zalogowanych],276:60 ConstructionanddetectionofcDNAlibraryofregeneratedliverfromChiloscylliumplagiosumZhaoYanjing, HuYanan,[link widoczny dla zalogowanych], LiuRui, WangYing, YuJianghe, YeBoping,[link widoczny dla zalogowanych], WangMin, WuWutong (SchoolofLifeScienceandTechnology, ChinaPharmaceuticalUniversity, Nanjing210009) AbstractThesharkmodelofliverregenerationwasconstructed, thetotalRNAwill8extractedfromthe24. hourregeneratedhepatictissues, andthemRNAwill8purifiedfurtherthroughthemagneticsubstancecombinedwithOligo (dT). Thepoly-dooxythymidinesandpoly-deoxycytidineswereintroducedintothe5'terminaland3'terminaloffirstcDNAstrandwiththehelpofM-MLVreversetranscriptaseandterminaltransferaserespectively. ThenthedoublestrandcDNAwiththere. stfictionsitesofBamHIandHindmwill8synthesizedthroughLD-PCR (1ong-distance-PCR) andligatedintopUC19vec. tor, andrecombinantvectorsweretransformedintoE. coliDI-ISctandamplified, thencDNAlibrarywill8analyzed. ThecapacityoftheconstructedcDNAlibrarywill8morethan4.92 × 105clonesperttgdoublestrandcDNA, andtherateofre. combinantwascalculatedasabout96.7%. Amongthe30clonesselectedrandomly. 25cDNAclonesweresequencedandblastedinGenBankanddbEST, mostofwhichwerenovelgenes, andmightplayanimportantroleonliverregenem. tion. WiththecDNAlibraryofsharkregeneratedhepatictissueeingsuccessfullyconstructed. itn1ightbeconvenienttocloneanddetectthedifferentiallydisplayedgenesexpressedduringliverregeneration. Keywords: shark, regeneratedhepatictissue,[link widoczny dla zalogowanych], cDNAlibrary a 86 -
相关的主题文章：
[link widoczny dla zalogowanych]
[link widoczny dla zalogowanych]
[link widoczny dla zalogowanych]
The post has been approved 0 times
|
|
