nlwxpearo
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 Posted: Thu 11:51, 10 Mar 2011 Post subject: Reticulated platelets and MPV, the relationship be |
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Reticulated platelets and MPV,[link widoczny dla zalogowanych], the relationship between the number of platelets
l,[link widoczny dla zalogowanych], plus 1OlPE-CD42b, 15min dark incubation,[link widoczny dla zalogowanych], add 1mlTO dye (final concentration 5g/m1),[link widoczny dla zalogowanych], dark incubated for 1h, the machine tests, the same time set the same type of negative control. Data analysis for the double standard method TO / PE ~ CD42b. 5 Statistical analysis using analysis of variance and linear correlation analysis. Results 1 to Coulter-JT of the MPV group reference value to detect 9O healthy individuals RP, the results in Table 1 (platelets were within normal range). RP reference value of the room 1O. 62 ± 1.56, three groups of age, gender differences were not statistically significant. Analysis of variance, large volume (MPV> 11.5f1) RP platelets were significantly higher than that of normal controls, the difference was significant (P 300 × 10. / L group and the normal group,[link widoczny dla zalogowanych], the difference was not statistically significant. Table 2 Number of different groups of RP PIT value () Pd0.01342 cases of low blood platelet count in patients with RP are shown in Table 3. RP patients blood was significantly higher than the normal x, LN (P <0.01), while the AA and ITP patients with RP value reached more than 2 times normal. Table 342 cases of RP in patients with blood value () to discuss the detection of the RP is currently no fixed methods and reference standards, very large differences in the scope of the report]. Therefore, the laboratories have established their own reference ranges, the reference value of RP Room 10.629 / 6 ± 1.569 / 6. RP and reticulocyte release from the bone marrow are immature cells ridicule, compared with normal platelets, the cytoplasm contains a small amount of mRNA, can be methylene blue staining, was coarse punctate distribution, with the mature platelets, mRNA gradually disappear. Thus RP can be more accurately reflected the situation in bone marrow platelet formation. MPV for the study of life and the conversion rates of platelet and platelet formation, mature, have a certain significance. In the past to reflect the multi-purpose MPV update status of platelets in peripheral blood, under normal circumstances, the smaller the plate age, the greater its volume. Based on the 90 patients with different MPV's RPJClinTransfusLabMed, ADr. 2004. Vol6. No. 2 detection can be seen: RP and MPV have a certain relationship, when the MPV is larger than normal, the RP was significantly higher, the difference was significant (P <0.01), that have more access to new and immature blood platelet . Koh [4 other studies found that bone marrow hematopoietic tissue of normal population, a large volume of platelets in the occurrence rate and the RP appearance rate was a significant positive correlation, we examined the MPV and RP had no significant correlation, possibly because of MPV reflect the average platelet volume , MPV increased only roughly reflect the emergence of a large increase in platelet volume. Platelet count is an important index reflecting the bleeding. When the platelet is less than 50 × 10. / L, may have bleeding symptoms. In this paper, platelets less than 100 × 10. / L, detection of healthy RP, found that the RP was significantly higher than that of normal controls. Excessive destruction of platelets in ITP patients, RP was significantly increased. May be due to the reduction of peripheral blood platelets can stimulate the accelerated release of bone marrow megakaryocytes platelets, platelet young students into the blood circulation, to supplement the loss of platelets. AML patients with AA and the formation of platelet barriers are reduced, RP value also increased significantly. The literature [4], when the absolute value of low platelets, RP absolute value is lower, the update rate of platelets was significantly decreased, reflecting the suppression of bone marrow function. MPV, RP reflects the update status of platelet formation, both of which have some relationship, MPV increases RP increased, but no significant correlation. When the platelet count decrease, RP increased, there is no significant correlation. Some blood for patients with thrombocytopenia, their RP values increased more significantly. Three for the diagnosis, treatment, monitoring is important, its affiliates be studied further.
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