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270 (4): 771 [10] Wei Jian Wen, Wu Yan, Zhong Xiaofen and so on. Marine functional genomics research. High-tech communications, 2OOO, 10 (10): 85lllJMichalopoulosGK,PUMA shoes wholesale, DeFrancesM. Liverregeneration. Sc / one. 1997,Wholesale Puma Shoes,276:60 ConstructionanddetectionofcDNAlibraryofregeneratedliverfromChiloscylliumplagiosumZhaoYanjing, HuYanan,ugg boots for sale, LiuRui, WangYing, YuJianghe, YeBoping,rosetta stone sale, WangMin, WuWutong (SchoolofLifeScienceandTechnology, ChinaPharmaceuticalUniversity, Nanjing210009) AbstractThesharkmodelofliverregenerationwasconstructed, thetotalRNAwill8extractedfromthe24. hourregeneratedhepatictissues, andthemRNAwill8purifiedfurtherthroughthemagneticsubstancecombinedwithOligo (dT). Thepoly-dooxythymidinesandpoly-deoxycytidineswereintroducedintothe5'terminaland3'terminaloffirstcDNAstrandwiththehelpofM-MLVreversetranscriptaseandterminaltransferaserespectively. ThenthedoublestrandcDNAwiththere. stfictionsitesofBamHIandHindmwill8synthesizedthroughLD-PCR (1ong-distance-PCR) andligatedintopUC19vec. tor, andrecombinantvectorsweretransformedintoE. coliDI-ISctandamplified, thencDNAlibrarywill8analyzed. ThecapacityoftheconstructedcDNAlibrarywill8morethan4.92 × 105clonesperttgdoublestrandcDNA, andtherateofre. combinantwascalculatedasabout96.7%. Amongthe30clonesselectedrandomly. 25cDNAclonesweresequencedandblastedinGenBankanddbEST, mostofwhichwerenovelgenes, andmightplayanimportantroleonliverregenem. tion. WiththecDNAlibraryofsharkregeneratedhepatictissueeingsuccessfullyconstructed. itn1ightbeconvenienttocloneanddetectthedifferentiallydisplayedgenesexpressedduringliverregeneration. Keywords: shark, regeneratedhepatictissue,wholesale PUMA, cDNAlibrary a 86 - 相关的主题文章： KART CAT Debt _3276 true religion jeans High-resolution SAR and optical image fusion of three-dimens sheepskin ugg boots Drying compressed air purifica |
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